Fabrication of cell pattern on poly(dimethylsiloxane) by vacuum ultraviolet lithography
Colloids and Surfaces B: Biointerfaces,
2010,
76,
381-385.
文章链接：http://dx.doi.org/10.1016/j.colsurfb.2009.11.013
干锦波同学在Colloids and Surfaces B: Biointerfaces发表研究论文
发布日期：2010-10-25
Cell patterning on substrates has played a significant role in the study of basic biology, cell-based biosensor and tissue engineering. In this report, a cell pattern was prepared on poly(dimethylsiloxane) (PDMS) substrate by vacuum ultraviolet (VUV) lithography. After immobilizing allyl-polyethylene glycol (APEG) onto PDMS, a chemical heterogeneous patterned surface was fabricated by VUV (Xe(2) excimer: 172nm) lithography with copper mesh as a photomask. The UV exposed domains can promote L929 cell adhesion and growth. However, non-exposed regions resist cell attachment because of the repelling property of PEG. Therefore, cell pattern could be achieved without pre-adsorption of cell adhesive species before cell culture.
